WP 5: Cellular and molecular effects of whole diet
Genetics will be studied in all the 1250 recruited subjects only before the dietary intervention. Telomere length will be studied in all the 1250 subjects before and after the dietary intervention. Epigenetics and “omics” as well as the assessment of proteasome/immunoprotesome composition and activity will be performed before and after the dietary intervention on the sub-group of 120 elderly subjects.
Finally, owing to the strict requirement of fresh blood and cells, in depth immune analysis will be performed before and after the dietary intervention on samples from 125 subjects undergoing the dietary intervention (not placebo).
The overall objectives of WP5 are to elucidate the molecular and cellular mechanisms of action of the whole diet on preventing of functional decline in elderly by using advanced immunology, genetic, epigenetic, biochemical as well as omics technologies.
Specific objectives are:
- To assess the effect of diet on inflammation and immunological status, function and regulation.
- To assess the effect of diet on metabolomics and lipidomics
- To assess the effect of diet on intestinal health, as measured by compositional and metagenomic analysis of the microbiome
- To assess genetic markers involved in inflammaging, and to assess the effect of diet on epigenetic, and chromosomal modifications